Table 1 Primers used for qRT-PCR
From: SET protein up-regulated testosterone production in the cultured preantral follicles
Gene | GeneBank ACC | Primer | Sequence(5′-3′) | Size |
|---|---|---|---|---|
SET | NM_023871 | Forward | CTTCAAAGTCCACCGAAATCAAATG | 146 bp |
(mouse) | Reverse | ACCTGCGTCAGAATGGTCAGTAAAC | ||
GAPDH | NM_008084 | Forward | AGGTTGTCTCCTGCGACTTCA | 187 bp |
(mouse) | Reverse | GGGTGGTCCAGGGTTTCTTACT | ||
StAR | http://NM_011485.4 | Forward | CCACCTGCATGGTGCTTCA | 142 bp |
(mouse) | Reverse | TTGGCGAACTCTATCTGGGTCTG | ||
CYP11A1 | NM_019779 | Forward | GGCACTTTGGAGTCAGTTTACAT | 186 bp |
(mouse) | Reverse | GTTTAGGACGATTCGGTCTTTCTT | ||
CYP17A1 | http://NM_007809.3 | Forward | TCTGGGCACTGCATCACG | 124 bp |
(mouse) | Reverse | GCTCCGAAGGGCAAATAACT | ||
HSD3B2 | http://NM_153193.2 | Forward | CTGCGATCCAGAAACCTTCC | 224 bp |
(mouse) | Reverse | TCTTCCTCTTGCACCAACAAC |