Figure 2From: SET protein up-regulated testosterone production in the cultured preantral folliclesEffect of SET knockdown on testosterone synthesis in theca cells. (A) Infection of follicular theca cells with recombinant adenoviruses. Follicles were infected with recombinant adenovirus AdSiRNA-SET or AdSiRNA-NS and cultured for 24āh. The majority of theca cells were infected as judged by the expression of GFP under a fluorescence microscope. Left: light microscope; right: fluorescence microscope (Scale barā=ā50āĪ¼m). (B) Western blot analysis of SET protein expression in follicles infected with recombinant adenovirus for 48āh. The levels of SET protein in follicles infected with AdSiRNA-SET were decreased about 43% compared with follicles infected with AdSiRNA-NS. (C) Effect of SET knockdown on theca cell testosterone synthesis. Compared with the follicles infected with AdSiRNA-NS, the amount of testosterone in the culture media of the AdSiRNA-SET infection group was significantly degraded. Results are presented as MeanāĀ±āSD from at least 3 independent experiments. Sample sizes were 20 follicles per treatment, per experiment. *, pā<ā0.05 compare with AdSiRNA-NS infected group.Back to article page