Figure 1

Effect of SET overexpression on testosterone synthesis in theca cells. (A) Infection of follicular theca cells with recombinant adenoviruses. Follicles were infected with recombinant adenovirus AdCMV-SET or AdCMV and cultured for 24 h. Over 90% theca cells were infected as judged by the expression of GFP under a fluorescence microscope. Left: light microscope; right: fluorescence microscope (Scale bar = 50 μm). (B) Western blot analysis of SET protein expression in follicles infected with recombinant AdCMV-SET adenovirus for 48 h. SET protein level in the follicles infected with AdCMV-SET were increased 1.8 times when compared with that in follicles infected with AdCMV. Tubulin was used as internal controls. (C) Effect of SET overexpression on testosterone synthesis in theca cells. Follicles were infected with AdCMV-SET or AdCMV for 48 h. Compared with that in the AdCMV infection group, testosterone production in the culture media of the AdCMV-SET infection follicles was significantly increased. Results are presented as Mean ± SD from at least 3 independent experiments. *, p < 0.05 compare with AdCMV.