In situ hybridization analyses of AMH ESR1 CCND1 and CDH2 mRNAs in male embryonic gonads during embryogenesis and testes from male chickens. Cell-specific localization of AMH, ESR1, CCND1 and CDH2 mRNAs was determined using in situ hybridization analysis. Cross-sections of right and left gonads from E6 to E18 male embryos, and testes from 12-week-old and adult chickens were hybridized with antisense or sense cRNA probes. Legend: M medullar region, C cortex region, Sc seminiferous cord, ST seminiferous tubules, S Sertoli cells. Scale bar represents 100 μm. See Methods for a complete description of the methods.