Figure 4From: Sex-specific expression of CTNNB1 in the gonadal morphogenesis of the chickenIn situ hybridization analyses of AMH ESR1 CCND1 and CDH2 mRNAs in male embryonic gonads during embryogenesis and testes from male chickens. Cell-specific localization of AMH, ESR1, CCND1 and CDH2 mRNAs was determined using in situ hybridization analysis. Cross-sections of right and left gonads from E6 to E18 male embryos, and testes from 12-week-old and adult chickens were hybridized with antisense or sense cRNA probes. Legend: M medullar region, C cortex region, Sc seminiferous cord, ST seminiferous tubules, S Sertoli cells. Scale bar represents 100 μm. See Methods for a complete description of the methods.Back to article page