Figure 2From: Sex-specific expression of CTNNB1 in the gonadal morphogenesis of the chickenComparison of relative expression and localization of CTNNB1 mRNA and protein in female embryonic gonads during embryogenesis and reproductive organs from female chickens. [A] Relative changes in CTNNB1 mRNA expression were determined using quantitative RT-PCR. The asterisks denote statistically significant differences (***P < 0.001). [B] Cell-specific localization of CTNNB1 mRNA was determined using in situ hybridization analysis. Cross sections of left gonad from E6 to E18 in female embryos, oviduct and ovary from 12-week-old and adult chickens were hybridized with antisense or sense cRNA probes. [C] Immunoreactive CTNNB1 protein during the development of chicken female gonad. For the IgG control, normal rabbit IgG was substituted for the primary antibody. Legend: M medullar region, C cortex region, Sc seminiferous cord, S Sertoli cells, LE luminal epithelium. Scale bar represents 100 μm. See Methods for a complete description of the methods.Back to article page