Figure 1From: Sex-specific expression of CTNNB1 in the gonadal morphogenesis of the chickenComparison of relative expression and localization of CTNNB1 mRNA and protein in male embryonic gonads during embryogenesis and reproductive organs from male chickens. [A] Relative changes in CTNNB1 mRNA expression were determined using quantitative RT-PCR. The asterisks denote statistically significant differences (***P < 0.001). [B] Cell-specific localization of CTNNB1 mRNA was determined using in situ hybridization analysis. Cross sections of left and right gonads from E6 to E18 in male embryos and testes from 12-week-old and adult chickens were hybridized with antisense or sense cRNA probes. [C] Immunoreactive CTNNB1 protein during the development of chicken male gonad. For the IgG control, normal rabbit IgG was substituted for the primary antibody. Legend: M medullar region, C cortex region, Sc seminiferous cord, S Sertoli cells. Scale bar represents 100 μm. See Methods for a complete description of the methods.Back to article page