Interaction of Fbxoo with its binding proteins identified by yeast two-hybrid screening. A. Colony-lift filter assays showing β-galactosidase activities in yeast cultures expressing both Fbxoo protein and its interacting proteins (Timp2 or Ak2 or H3f3a). B-D. Yeast β-galactosidase liquid assays confirming interactions between Fbxoo and its binding proteins including Timp2 (B), Ak2 (C) and H3f3a (D). Error bars indicate standard deviation (n=3). Astrisk indicates a significant difference (P<0.05).