Figure 4

Localization of fbxoo mRNA and protein in oocytes of different developmental stages. A. In situ hybridization analysis using antisense RNA probe showing predominant expression of fbxoo mRNA in early stage oocytes. B. A control section hybridized with the sense probe showing no significant hybridization signal. D. Detection of strong Fbxoo protein expression signal in early pre-vitellogenic and early vitellogenic oocytes by immunohistochemistry using anti-Fbxoo antibody. E. A negative control section without primary antibody. C and F. Ovarian sections stained with Hematoxylin and Eosin to show oocytes of different vitellogenic stages. EV: early vitellogenic oocyte; EpV: early pre-vitellogenic oocyte. The arrow indicates granulosal cell layer. The scale bars indicate 300 μm.