Expression of fbxoo mRNA and protein during ovarian and embryonic development. A. Quantitative analysis of fbxoo mRNA expression during ovarian development and early embryogenesis by real time PCR. Ovarian samples from different developmental stages including early pre-vitellogenesis (EpV), late pre-vitellogenesis (LpV), early vitellogenesis (EV), mid-vitellogenesis (MV) and late-vitellogenesis (LV) were used in the analysis. B. Western blot analysis of Fbxoo protein expression in ovaries of different developmental stages and selected somatic tissues including muscle (Mu), spleen (Sp) and liver (Li). Rainbow trout β-actin (Actb) serves as a control.