Table 1 Primers used for the full-length cloning and expression of the porcine MAP1LC3A gene
Primer name | Sequence | Size |
|---|---|---|
cDNA cloning primers | ||
GeneRacer 3 ′ -primer | 5 ′ GCTGTCAACGATACGCTACGTAACG 3 ′ | |
GeneRacer 3 ′ -nested primer | 5 ′ CGCTACGTAACGGCATGACAGTG 3 ′ | |
GeneRacer 5 ′ -primer | 5 ′ CGACTGGAGCACGAGGACACTGA 3 ′ | |
GeneRacer 5 ′ -nested primer | 5 ′ GGACACTGACATGGACTGAAGGAGTA 3 ′ | |
5 ′ RACE-Rv primer 1 | 5 ′ TGTCCAGGACTGGCAGCTGCTTCT 3 ′ | |
5 ′ RACE-Rv primer 2 | 5 ′ CCAACTCGCTCATGTTGACATGGT 3 ′ | |
3 ′ RACE-Fw primer | 5 ′ ATCGAGCGCTACAAGGGTGAGAAGC 3 ′ | |
Gene expression primers | ||
Porcine GAPDH Fw | 5 ′ CCCGTTCGACAGACAGCCGTG 3 ′ | 238 |
Porcine GAPDH Rv | 5 ′ CCGCCTTGACTGTGCCGTGG 3 ′ | |
Porcine MAP1LC3A Fw | 5 ′ AGAAGCAGCTGCCAGTCCTGGACA 3 ′ | 687 |
Porcine MAP1LC3A Fw | 5 ′ CAGGCAGGCCTGAGCAATCTTTATT 3 ′ | |
Porcine ATG5 Fw | 5 ′ AGAGAAGTCTGTCCTTCCGCAGTCG 3 ′ | 241 |
Porcine ATG5 Rv | 5 ′ AAGCAGAAGGGTGACATGCTCTGGT 3 ′ | |