Effect of rapamycin treatment on the expression of MAP1LC3A and ATG5 protein expression in cultured granulosa cells. A. Immunofluorescence staining of cultured granulosa cells. B. Western blot analysis of the porcine MAP1LC3A protein. C. Real-time PCR of porcine MAP1LC3A mRNA. Data represent the mean ± standard error of 3 individual experiments (p < 0.05).