In situ detection and expression analyses of MAP1LC3A mRNA in the ovary. A. In situ hybridization of MAP1LC3A mRNA in the Graafian follicle of normal and miniature pigs. Arrows indicate positive cells. GC: granulosa cells; TC: theca cells. Prehybridization solution was used as the control for the Graafian follicle below the negative panel. B. Reverse transcription PCR and C. Real-time PCR of total RNAs from normal and miniature pig ovaries. Experiments were repeated 3 times, and data are expressed as mean ± standard error (p < 0.05). Black bar=100 um in all figures.