Figure 5

Type of inhibition and docking of steroids to ASCE and ASWT proteins. The new calculated kinetic enzyme parameters obtained in the presence of the corresponding IC50 concentration of progesterone for each enzyme were Km = 2.451 μM and Vmax = 37.91 μM/24 h for ASCE, and Km = 10.78 μM and Vmax = 51.61 μM/24 h for ASWT. Progesterone inhibits the aldosterone synthase wild type in a competitive fashion. (A and D). Docking experiments show that DOC binds in a similar mode to ASCE compared to ASWT (B and E). Progesterone also has a similar binding mode to ASCE and ASWT, but penetrates deeper into the ASCE pocket (C and F).