Figure 4

Molecular modelling of CYP11B1 and ASCE proteins. Schematic representation of the secondary structures and electrostatic potential profiles of CYP11B1 (11BH, Panel A), CYP11B1/B2 (ASCE, Panel B) and CYP11B2 (ASWT, Panel C). The ASCE secondary structure is colored according to crossover occurring at helix G, where grey represents the CYP11B1 portion and green represents the CYP11B2 portion of the chimeric enzyme. The solvent accessible surface colored according to the calculated electrostatic potential shows that the steroid binding site in ASCE is more electronegative and more extended than that of the ASWT (553.62 v/s 471.87 Å³).