Expression of aldosterone production by ASCE and ASWT in vitro. A) Schematic representation of the CYP11B1/B2 chimeric gene, showing the crossover region between intron 3 of CYP11B1 and exon 4 of CYP11B2. B) A representative image of the transfection efficiency. Green fluorescence (upper panel) and the same bright field (lower panel) of transfected or non-transfected HEK-293 (NT). C) An average transfection efficiency of 50 percent was observed from three independent experiments for each construct. D) A representative Western blot of CYP11B2. Line 1, human adrenocortical adenoma; line 2, NT-HEK-293; line3, PCMV-CYP11B1 transfected HEK-293; line 4, PCMV-CYP11B1/B2; and line 5, PCMV-CYP11B2 transfected HEK-293. Actin was used as a loading control.