In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system

Table 2 Number of GC recovered and the percentage of samples lost due to visible blood contamination, low number of GC (<100,000 cells), or E2/P4 ratio lower than 1

End Point	Hosltein	Gir
Number of GC recovered	615,054 ± 58,122^a	450,095 ± 36,047^b
Total RNA extracted (ng/μL)	8.8 ± 0.7	9.8 ± 1.7
% of samples lost due to:
Visible blood contamination	11.4% (5/44)^a	14.9% (11/77)^a
Low number of GC	4.5% (2/44) ^a	3.9% (3/77) ^a
E2/P4 lower than 1	4.5% (2/44)^a	3.9% (3/77)^a

^{a, b}The means without a common superscript in the same column differed by Tukey’s test (P < 0.05).

ISSN: 1477-7827