In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system

Table 1 Recovery efficiency in vitro using a conventional (80 cm Teflon tubing) or an adapted (with 0.25 mL semen straws) aspiration system

Equivalent follicle diameter (mm)	Expected FF volume (mL)	Recovery efficiency (%)
		Conventional OPU system (n = 5)	Adapted system¹
			(n = 5)
12	0.90	87.3 ± 1.1^a	-
10	0.52	80.1 ± 2.5^a	-
8	0.27	45.6 ± 4.0^b	78.3 ± 3.3^a
6	0.11	12.4 ± 4.3^c	75.6 ± 4.0^a
4	0.03	0.0 ± 0.0^d	73.9 ± 5.0^a

^{a, b, c, d}The means without a common superscript in the same column differed by Tukey’s test (P < 0.05).
¹ The adapted system was used only for 8, 6, and 4 mm follicles.

ISSN: 1477-7827