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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Low-dose monobutyl phthalate stimulates steroidogenesis through steroidogenic acute regulatory protein regulated by SF-1, GATA-4 and C/EBP-beta in mouse Leydig tumor cells

Figure 4

Expression of transcription factors protein in MBP treated MLTC-1 cells. Cells were treated with MBP (10(−9), 10(−8), 10(−7), or 10(−6) M) or DMSO as control for 24 h, and then stimulated with hCG for 4 h. The panel shows a representative western blot analysis of SF-1, GATA-4, C/EBP-beta and DAX-1 protein expression corrected by corresponding GAPDH. SF-1, GATA-4, C/EBP-beta and DAX-1 protein expressions were determined by quantified bar graph using GAPDH. * indicates significant difference when the values were compared with the control (P< 0.05).

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