Examination of transcription factors binding in MBP treated MLTC-1 cells. (A-C) The binding of SF-1, GATA-4, and C/EBP-beta with the StAR promoter regions after MBP treatment for 24 h, and then stimulated with hCG for 4 h. It was evaluated by an EMSA described in the “Materials and Methods” section. Lane 1: blank (only labelled probe); Lane 2: control-without MBP treatment (labelled probe and nuclear protein); Lane 3: treated with 10(−9) M MBP for 24 h (labelled probe and nuclear protein); Lane 4: treated with10 (−6) M MBP for 24 h (labelled probe and nuclear protein); Lane 5: positive control (unlabeled probe, labelled probe, and nuclear protein). The DNA-binding of SF-1, GATA-4 and C/EBP-beta with the StAR promoter regions was determined by quantified bar graph using control without MBP treatments. * indicates significant difference when the values were compared with control (P<0.05).