Figure 2

Expression of StAR, P450scc and 3 beta-HSD in MBP treated MLTC-1 cells. Cells were treated with MBP (10(−9), 10(−8), 10(−7), or 10(−6)M) or DMSO as control for 24 h and then stimulated with hCG for 4 h. (A) StAR mRNA levels were determined by quantitative real-time RT-PCR using GAPDH as housekeeping gene. (B) The panel shows a representative western blot analysis of StAR protein expression corrected by corresponding GAPDH. The figure represents as mean ± S.E. of StAR protein integrated optical density corrected by corresponding GAPDH. Each data point was normalized to the control (DMSO) and represents as mean ± S.E. from three independent experiments. * indicates significant difference when the values were compared with the control (P< 0.05). (C-E) The panel shows a representative western blot analysis of P450scc and 3 beta-HSD protein expression corrected by corresponding GAPDH. The figure represents as mean ± S.E. of P450scc and 3 beta-HSD protein integrated optical density corrected by corresponding GAPDH. Each data point was normalized to the control (DMSO) and represents as mean ± S.E. from three independent experiments.