Figure 1

Analysis of cell viability and production of progesterone in MBP treated MLTC-1 cells. (A) Cells were cultured with various concentrations of MBP (10(−9), 10(−8), 10(−7), or 10(−6) M) or DMSO as control for 24 h. At the end of incubation, the cell viability was determined using MTT assay. Results were presented as percentage of the values measured in untreated control cells. Each data point represented as mean ± S.E. from three separate experiments in which treatments were performed in quadruplicate. No statistically significant difference was found between the treatment groups and the control group. (B) Cells were treated with various concentrations of MBP (10(−9), 10(−8), 10(−7), or 10(−6) M) or DMSO as control for 24 h, and then the medium was changed to SFM and cells were stimulated with hCG (0.1 U/ml) for 4 h. Each data point represents as mean ± S.E. from at least three separate experiments in which treatments were performed in quadruplicates. * indicates significant difference when the values were compared with the control (P< 0.05).