Real-time quantitative RT-PCR assays. Assays were performed to determine the relative mRNA expression levels of (A) GOAT (MBOAT4), (B) PC1/3 (PCSK1), (C) PC2 (PCSK2), and (D) furin (FURIN) in the RWPE-1 and RWPE-2 normal-prostate derived cell lines and the DU145, LNCaP and PC3 prostate cancer-derived cell lines. Data are expressed relative to the RWPE-1 cell line mRNA levels (set as 1) and are presented as means ± S.E.M. (n = 2, assays performed in duplicate) and compared by one-way ANOVA, followed by Tukey’s post-hoc analysis.* = P < 0.05 versus the RWPE-1 cell line.