Figure 4
From: Superovulation induces defective methylation in line-1 retrotransposon elements in blastocyst
Expression of Dnmts in zygotes derived from control and superovulated females. qRT-PCR was applied to evaluate the mRNA abundance of A) Dnmt1o, B) Dnmt3a, C) Dnmt3b and D) Dnmt3L in zygotes derived from control, low- (6 IU) and high-dose hormone treatment groups, respectively. Data were normalized to the average levels of Gapdh and Atp5 for each sample. The fold change was calculated using the comparative CT method. Data are presented as mean ± SD.