Figure 3
From: Superovulation induces defective methylation in line-1 retrotransposon elements in blastocyst
Expression of Dnmts in MII oocytes derived from control and superovulated females. qRT-PCR was applied to evaluate the mRNA abundance of A) Dnmt1o, B) Dnmt3a, C) Dnmt3b and D) Dnmt3L in MII oocytes derived from control, low- (6 IU) and high-dose hormone treatment groups, respectively. Two reference genes, Gapdh and Atp5, were used for each sample. Data were normalized to the average levels of the two reference genes. The fold change was calculated using the comparative CT method. Data are presented as mean ± SD.