Figure 3
CAPN 7 overexpression upregulates MMP-2 expression and activity in hESC. (A) MMP-2 mRNA expression was measured using real-time PCR following CAPN 7 overexpression; * P < 0.05 compared to Ad-LacZ. (B, C) MMP-2 protein expression and activity were measured using western blotting and zymography, respectively, following CAPN 7 overexpression. (D) OA-Hy decreased the migratory effects of CAPN 7 overexpression on hESC by approximately 50% at all time points; ** P < 0.01 compared to controls. (E) OA-Hy decreased the invasion ability of CAPN 7 overexpression in hESC by approximately 55%; ** P < 0.01 compared to the control. (F) TIMP-2 mRNA expression was measured using real-time PCR following CAPN 7 overexpression; * P < 0.05 compared to Ad-LacZ. (G) The ratio of MMP-2 to TIMP-2 mRNA expression was measured under the same conditions, * P < 0.05 compared to controls. (H) A coimmunoprecipitation assay was used to detect interactions between CAPN 7 and AP-2α (IP: immunoprecipitation; WB: western blotting) (hESC were isolated from normal fertile women, n = 18).