CAPN 7 affects hESC migration and invasion. (A) HESC were infected with Ad-LacZ or Ad-Flag-CAPN 7 (MOI = 50). The migration rates (migration distance from the 0 h time point to a certain time) of the two groups are shown in the line graph; **P < 0.01 compared with Ad-LacZ. (B) Migration rates of hESC transfected with control siRNA (siCTL) or siRNA specific for CAPN 7 (50 nM); * P < 0.05, **P < 0.01 compared with siCTL. (C) CAPN 7 overexpression increased cell invasion by approximately 2-fold, * P < 0.05 compared to Ad-LacZ. (D) Knockdown of endogenous CAPN 7 expression decreased cell invasion by approximately 65% compared with siCTL (* P < 0.05). CAPN 7 protein expression was measured by western blotting (hESC were isolated from normal fertile women, n = 9).