Figure 4
From: Temporo-spatial expression of adrenomedullin and its receptors in the bovine placenta
mRNA localization of AM , CRLR , RAMP2 and RAMP3 in the bovine placentome on day 150 of gestation. (A, D, G and J) Digoxigenin (DIG)-labeled anti-sense cRNA probes were used. (B, E, H and K) enlarged images of frames in A, D, G and J, respectively. (C, F, I and L) DIG-labeled sense cRNA probes were used. Sections (7 μm) of bovine placentome on Day 150 of gestation were hybridized with each probe. AM mRNA (A and B) was detected in only BNCs. mRNA expression of CRLR (D and E), RAMP2 (G and H) and RAMP3 (J and K) was detected in cotyledonary villous and CEs. BNC, trophoblast binucleate cell; CE, caruncular epithelial cell; CV, cotyledonary villous. Scale bars = 20 μm.