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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Temporo-spatial expression of adrenomedullin and its receptors in the bovine placenta

Figure 3

mRNA localization of AM , CRLR , RAMP2 and RAMP3 in the bovine placentome on day 56 of gestation. (A, D, G and J) Digoxigenin (DIG)-labeled anti-sense cRNA probes were used. (B, E, H and K) enlarged images of frames in A, D, G and J, respectively. (C, F, I and L) DIG-labeled sense cRNA probes were used. Sections (7 μm) of bovine placentome on Day 56 of gestation were hybridized with each probe. AM mRNA (A and B) was detected in only in BNCs. mRNA expression of CRLR (D and E) , RAMP2 (G and H) and RAMP3 (J and K) were detected in cotyledonary villous and CEs. BNC, trophoblast binucleate cell; CE, caruncular epithelial cell; CV, cotyledonary villous. Scale bars = 20 μm.

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