Microarray (A) and qPCR (B) analysis of IFNτ-stimulated gene expression in PBL. (A) Blood was collected from cows on days 0 (n = 5), 14 (n = 3), and 21 (n = 5) after artificial insemination (AI). Three and five cows samples were anlyzed on day 14 and 21 of gestation, respectively. Each sample was applied to an oligo microarray. Data are shown as mean ± SEM. (B) Open circles represent estrous cycle cows (control, n = 5). Crosses and solid circles represent infertile cows (n = 8) and pregnant cows (n = 6), respectively, which were subjected to AI on day 0 and diagnosed via ultrasonography. Data are shown as mean ± SEM. The copy number of each mRNA is indicated to confirm the minimum amount of mRNA produced. Values significantly different from the value on day 0 of gestation (before AI) are shown with an asterisk. P values less than 0.05 were considered significant. d: day of gestation and/or estrous cycle.