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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner

Figure 4

Quantitative real-time RT-PCR analyses for efferent duct ligation (EDL) to determine the involvement of testicular factors in regulating Spag11a . The efferent ducts connecting the testis and the epididymis were ligated to prevent testicular fluid from entering the epididymis. Total caput epididymal RNA from both sides were isolated at 6 h, 1 d, 3 d and 5 d after the ligation. Unligated epididymis was used as a control (Co). Total RNA from each group was analyzed by quantitative real-time RT-PCR. The expression level in each group was normalized to beta-actin. The results showed that Spag11a expression was not affected at 6 h to 1 d. after the ligation. Spag11a was transiently up-regulated at 3 d ligation (P < 0.01) before returning to the normal level at day 5. The error bars represent the SEM (n = 3).

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