Skip to main content

Table 1 Dose–response effect of the GM-CSF on nuclear maturation of bovine oocytes matured in vitro

From: Granulocyte-macrophage colony stimulating factor (GM-CSF) enhances cumulus cell expansion in bovine oocytes

Treatment COC GV GVBD MI MII
  (n) (%) (%) (%) (%)
SOF 75 11/78 (14.6) 9/75 (12.0) 14/75 (18.7) 41/75 (54.7)
SOF + GM-CSF (1 ng/ml) 71 11/71 (15.5) 5/71 (7.0) 13/71 (18.3) 42/71 (59.2)
SOF + GM-CSF (10 ng/ml) 59 8/59 (13.6) 4/59 (6.8) 15/59 (25.4) 32/59 (54.2)
SOF + GM-CSF (100 ng/ml) 89 14/89 (15.7) 4/89 (4.5) 18/89 (20.2) 53/89 (59.6)
TCM 95 1/95 (1.0) 4/95 (4.2) 11/95 (11.6) 79/95 (83.2*)
  1. GV Germinal Vesicle, GVBD Geminal Vesicle Break-Down, MI metaphase I.
  2. MII metaphase II. (*) Indicate significant differences between treatments (P < 0.05).
  3. (There were 3 replicates for this experiment).