Figure 5

Relative expression of IGF-2 gene transcript. (mean ± SEM) in bovine cumulus cells (black bars) and oocytes (grey bars) in COC (n = 30/per group) in vitro matured in TCM, SOF alone or supplemented with 100 ng/ml of GM-CSF. (*) Indicate significant differences compared with immature control (IC). Different superscripts (a,b,c) indicate significant (P < 0.05) difference.