Table 2 Protein name, NCBI database index number, calculated MW, pI, peptide coverage and Mascot score in ROS- group
From: Proteomic analysis of human spermatozoa proteins with oxidative stress
No. | Protein name | NCBI database index number | Calculated MW (kDa) | pI | LTQ | |
|---|---|---|---|---|---|---|
Peptides (Coverage) | Mascot score | |||||
1 | A-Kinase anchor protein 4 isoform 2 | 21493039 | 94 | 6.7 | 34 (54%) | 11284 |
2 | lactotransferrin precursor | 54607120 | 80 | 8.5 | 32 (56%) | 6049 |
3 | gyceraldehyde-3-phosphate dehydrogenase, | 7657116 | 44 | 8.4 | 12 (48%) | 5959 |
4 | spermatogenic tubulin, beta, 2 precursor | 5174735 | 50 | 4.7 | 25 (68%) | 5842 |
5 | semenogelin I isoform a preproprotein | 4506883 | 52 | 9.3 | 11(24%) | 4965 |
6 | tubulin, beta 4 | 21361322 | 50 | 4.8 | 19 (58%) | 4879 |
7 | semenogelin II precursor | 4506885 | 65 | 9.1 | 15 (25%) | 4205 |
8 | tubulin, alpha 3c | 17921993 | 50 | 4.9 | 15 (56%) | 2645 |
9 | ropporin, rhophilin associated protein 1B | 59891409 | 24 | 5.1 | 6 (53%) | 2427 |
10 | prolactin-induced protein | 4505821 | 16 | 8.2 | 8 (62%) | 2164 |
11 | A-kinase anchor protein 3 | 21493041 | 95 | 5.8 | 16 (28%) | 1800 |
12 | outer dense fiber of sperm tails 2 Isoform 2 | 24430183 | 73 | 7.2 | 16 (31%) | 1722 |
13 | sperm protein associated with the Nucleus, X chromosome, family Member C | 13435137 | 11 | 5.0 | 3 (45%) | 1437 |
14 | tubulin alpha 6 | 14389309 | 50 | 4.9 | 11 (45%) | 1374 |
15 | ropporin | 21359920 | 24 | 5.5 | 6 (43%) | 1280 |
16 | clusterin isoform 1 | 42716297 | 58 | 6.2 | 5 (15%) | 1257 |
17 | sorbitol dehydrogenase | 156627571 | 38 | 8.2 | 4 (23%) | 1249 |
18 | beta actin | 4501885 | 42 | 5.2 | 6 (34%) | 1232 |
19 | pyruvate kinase, muscle isoform M1 | 3328642 | 58 | 7.6 | 13 (47%) | 1133 |
20 | histone cluster 1, H2aa | 25092737 | 14 | 10.8 | 4 (51%) | 1121 |
21 | mitochondrial ATP synthase beta subunit precursor | 32189394 | 56 | 5.2 | 13 (38%) | 1031 |
22 | fatty acid synthase | 41872631 | 275 | 6.0 | 10 (9%) | 935 |
23 | glutathione S-transferase mu 3 | 23065552 | 26 | 5.3 | 7 (32%) | 929 |
24 | histone cluster 1, H2ba -1 peptide | 24586679 | 14 | 10.3 | 1 (11%) | 823 |
25 | fibronectin 1 isoform 3 preproprotein | 16933542 | 262 | 5.4 | 16 (11%) | 790 |
26 | L-lactate dehydrogenase C | 4504973 | 36 | 7.0 | 2 (7%) | 693 |
27 | heat shock 90Â kDa protein 1, alpha isoform 2 | 154146191 | 85 | 4.9 | 10 (18%) | 682 |
28 | glyceraldehyde-3-phosphate dehydrogenase | 7669492 | 36 | 8.5 | 3 (15%) | 621 |
29 | sperm autoantigenic protein 17 | 8394343 | 17 | 4.7 | 2 (22%) | 552 |
30 | triosephosphate isomerase 1 isoform 1 | 4507645 | 26 | 6.4 | 6 (61%) | 530 |
31 | mitochondrial malate dehydrogenase precursor | 21735621 | 35 | 8.9 | 5 (23%) | 512 |
32 | phosphoglycerate kinase 2 | 31543397 | 45 | 8.7 | 4 (17%) | 506 |
33 | heat shock 70Â kDa protein 2 | 13676857 | 70 | 5.5 | 8 (22%) | 423 |
34 | histone cluster 1, H2ae | 10645195 | 14 | 11.0 | 3 (43%) | 392 |
35 | acetyl-Coenzyme A acetyltransferase 1 precursor | 4557237 | 45 | 8.9 | 8 (34%) | 388 |
36 | eukaryotic translation elongation factor 1 alpha 1 | 4503471 | 50 | 9.1 | 7 (30%) | 383 |
37 | ATP synthase, H + transporting, mitochondrial F1 complex, alpha subunit precursor | 4757810 | 59 | 9.1 | 8 (22%) | 378 |
38 | outer dense fiber of sperm tails 1 | 194248060 | 30 | 8.4 | 3 (22%) | 334 |
39 | voltage-dependent anion channel 2 | 42476281 | 32 | 7.4 | 3 (19%) | 332 |
40 | acid phosphatase, prostate short isoform precursor | 6382064 | 44 | 5.8 | 5 (16%) | 326 |
41 | brain creatine kinase | 21536286 | 42 | 5.3 | 7 (29%) | 268 |
42 | saccharopine dehydrogenase (putative) | 55770836 | 47 | 9.2 | 4 (29%) | 265 |
43 | protein disulfide-isomerase A3 precursor | 21361657 | 57 | 5.9 | 2 (9%) | 261 |
44 | eukaryotic translation elongation factor 2 | 4503483 | 96 | 6.4 | 3 (8%) | 261 |
45 | heat shock protein 90Â kDa beta, member 1 | 4507677 | 92 | 4.7 | 5 (8%) | 250 |
46 | heat shock protein beta-1 | 4504517 | 22 | 5.9 | 4 (36%) | 191 |
47 | glutamine synthetase | 19923206 | 42 | 6.4 | 3 (14%) | 179 |
48 | valosin-containing protein | 6005942 | 89 | 5.1 | 7 (18%) | 177 |
49 | phospholipase A2, group IIA precursor | 4505849 | 16 | 9.4 | 2 (23%) | 170 |
50 | tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide | 4507953 | 27 | 4.7 | 2 (19%) | 167 |
51 | L-lactate dehydrogenase A isoform 1 | 5031857 | 36 | 8.4 | 2 (7%) | 167 |
52 | fructose-bisphosphate aldolase A | 4557305 | 39 | 8.3 | 3 (16%) | 167 |
53 | chaperonin containing TCP1, subunit 5 (epsilon) | 24307939 | 60 | 5.4 | 3 (9%) | 157 |
54 | voltage-dependent anion channel 3 isoform b | 25188179 | 30 | 8.8 | 4 (26%) | 147 |
55 | peroxiredoxin 6 | 4758638 | 25 | 6.0 | 4 (33%) | 140 |
56 | glutathione peroxidase 4 isoform A precursor | 75709200 | 22 | 8.6 | 5 (43%) | 139 |
57 | transglutaminase 4 (prostate) | 156627577 | 77 | 6.3 | 5 (9%) | 136 |
58 | enolase 1 | 4503571 | 47 | 7.0 | 3 (12%) | 134 |
59 | T-complex protein 1 isoform b | 57863259 | 44 | 7.5 | 5 (30%) | 129 |
60 | CSE1 chromosome segregation 1-like protein | 29029559 | 111 | 5.5 | 2 (3%) | 128 |
61 | fumarate hydratase precursor | 19743875 | 54 | 8.8 | 3 (12%) | 115 |
62 | lectin, mannose-binding 2 precursor | 5803023 | 40 | 6.4 | 2 (13%) | 115 |
63 | clathrin heavy chain 1 | 4758012 | 193 | 5.4 | 6 (7%) | 111 |
64 | chaperonin containing TCP1, subunit 4 (delta) | 38455427 | 58 | 7.9 | 4 (19%) | 107 |
65 | RAB2A, member RAS oncogene family | 4506365 | 23 | 6.0 | 3 (18%) | 106 |
66 | chromosome 20 open reading frame 3 | 24308201 | 46 | 5.8 | 3 (19%) | 105 |