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Table 1 Protein name, NCBI database index number, calculated MW, pI, peptide coverage and Mascot score in ROS+ group

From: Proteomic analysis of human spermatozoa proteins with oxidative stress

No.

Protein name

NCBI database index number

Calculated MW (kDa)

pI

LTQ

Peptides

Mascot

(Coverage)

score

1

A-Kinase anchor protein 4 isoform 2

21493039

94

6.7

34 (54%)

10988

2

lactotransferrin precursor

54607120

80

8.5

36 (64%)

7287

3

tubulin, beta, 2

5174735

50

4.7

24 (68%)

6702

4

tubulin, beta 4

21361322

50

4.8

19 (57%)

5071

5

glyceraldehyde-3-phosphate dehydrogenase, spermatogenic

7657116

44

8.4

12 (48%)

5711

6

tubulin, alpha 3c

17921993

50

4.9

15 (51%)

2942

7

ropporin, rhophilin associated protein 1B

59891409

24

5.1

7 (56%)

2587

8

semenogelin II precursor

4506885

65

9.1

13 (22%)

2277

9

outer dense fiber of sperm tails 2 isoform 2

24430183

73

7.2

16 (31%)

2051

10

semenogelin I isoform a preproprotein

4506883

52

9.3

12 (31%)

1983

11

sperm protein associated with the nucleus, X chromosome, family member C

13435137

11

5.0

3 (45%)

1892

12

tubulin, alpha, ubiquitous

57013276

50

4.9

13 (46%)

1616

13

glutathione S-transferase mu 3

23065552

26

5.3

7 (37%)

1595

14

prolactin-induced protein

4505821

16

8.2

8 (62%)

1559

15

A Kinase anchor protein 3

21493041

95

5.8

20 (35%)

1416

16

histone cluster 1, H2ba

24586679

14

10.3

1 (11%)

1371

17

mitochondrial malate dehydrogenase precursor

21735621

35

8.9

8 (33%)

1342

18

histone cluster 1, H2aa

25092737

14

10.8

6 (58%)

1334

19

clusterin isoform 1

42716297

58

6.2

6 (16%)

1204

20

ropporin

21359920

24

5.5

6 (43%)

1184

21

sorbitol dehydrogenase

156627571

38

8.2

7 (35%)

1095

22

mitochondrial ATP synthase beta subunit precursor

32189394

56

5.2

16 (53%)

976

23

beta actin

4501885

42

5.2

9 (54%)

909

24

triosephosphate isomerase 1 isoform 1

4507645

26

6.4

9 (55%)

761

25

glyceraldehyde-3-phosphate dehydrogenase

7669492

36

8.5

6 (32%)

706

26

L-lactate dehydrogenase C– 1 peptide

4504973

36

7.0

1 (5%)

674

27

fatty acid synthase

41872631

275

6.0

3 (8%)

669

28

sperm autoantigenic protein 17

8394343

17

4.7

2 (22%)

592

29

pyruvate kinase, muscle isoform M2

33286418

58

7.9

11 (38%)

560

30

ATP synthase, H + transporting, mitochondrial F1 complex, alpha subunit precursor

4757810

59

9.1

8 (23%)

542

31

brain creatine kinase

21536286

42

5.3

7 (32%)

511

32

heat shock protein 90 kDa beta, member 1

4507677

92

4.7

6 (10%)

502

33

heat shock 70 kDa protein 2

13676857

70

5.5

10 (21%)

448

34

heat shock 90 kDa protein 1, alpha isoform 1

153792590

98

5.0

8 (13%)

447

35

saccharopine dehydrogenase (putative)

55770836

47

9.2

5 (29%)

395

36

actin, alpha 1, skeletal muscle

4501881

42

5.2

6 (36%)

374

37

glutamine synthetase

19923206

42

6.4

3 (9%)

374

38

fructose-bisphosphate aldolase A

4557305

39

8.3

5 (29%)

366

39

eukaryotic translation elongation factor 1 alpha 1

4503471

50

9.1

8 (36%)

347

40

fibronectin 1 isoform 3 preproprotein

16933542

262

5.4

6 (4%)

321

41

prostate, ovary, testis expressed protein on chromosome 2

153791352

123

5.8

4 (8%)

317

42

acetyl-Coenzyme A acetyltransferase 1 precursor

4557237

45

8.9

9 (33%)

316

43

voltage-dependent anion channel 2

42476281

32

7.4

4 (22%)

293

44

outer dense fiber of sperm tails 1

194248060

30

8.4

4 (26%)

283

45

phosphoglycerate kinase 2

31543397

45

8.7

5 (30%)

253

46

histone cluster 1, H2ae

10645195

14

11.0

4 (49%)

251

47

protein disulfide – isomerase A3 precursor

21361657

57

5.9

4 (13%)

243

48

acid phosphatase, prostate short isoform precursor

6382064

44

5.8

5 (16%)

240

49

voltage-dependent anion channel 3 isoform b

25188179

30

8.8

5 (27%)

226

50

phospholipase A2, group IIA precursor

4505849

16

9.4

2 (23%)

202

51

transglutaminase 4 (prostate)

156627577

77

6.3

8 (17%)

187

52

glutathione peroxidase 4 isoform A precursor

75709200

22

8.6

4 (32%)

181

53

heat shock 70 kDa protein 5

16507237

72

5.0

4 (8%)

161

54

heat shock protein beta-1

4504517

22

5.9

3 (31%)

160

55

angiotensin I converting enzyme 1 isoform 1 precursor

4503273

150

5.9

3 (4%)

160

56

peroxiredoxin 6

4758638

25

6.0

2 (16%)

145

57

chaperonin containing TCP1, subunit 5 (epsilon)

24307939

60

5.4

4 (12%)

142

58

valosin-containing protein

6005942

89

5.1

3 (7%)

142

59

sperm acrosomal membrane protein 14 – 1 peptide

19424138

13

5.4

1 (11%)

132

60

chaperonin containing TCP1, subunit 8 (theta)

48762932

60

5.4

4 (14%)

131

61

L-lactate dehydrogenase A isoform 1

5031857

36

8.4

2 (13%)

124

62

phosphoglycerate dehydrogenase

23308577

57

6.2

3 (13%)

112

63

clathrin heavy chain 1

4758012

193

5.4

4 (5%)

108

64

chaperonin containing TCP1, subunit 4 (delta)

38455427

58

7.9

5 (21%)

99

65

peptidylprolyl isomerase A

10863927

18

7.6

2 (23%)

96

66

fumarate hydratase precursor

19743875

54

8.8

8 (31%)

92

67

RAB2A, member RAS oncogene family

4506365

23

6.0

3 (18%)

92

68

olfactomedin 4 precursor

32313593

57

5.5

2 (5%)

90