From: Proteomic analysis of human spermatozoa proteins with oxidative stress
No. | Protein name | NCBI database index number | Calculated MW (kDa) | pI | LTQ | |
---|---|---|---|---|---|---|
Peptides | Mascot | |||||
(Coverage) | score | |||||
1 | A-Kinase anchor protein 4 isoform 2 | 21493039 | 94 | 6.7 | 34 (54%) | 10988 |
2 | lactotransferrin precursor | 54607120 | 80 | 8.5 | 36 (64%) | 7287 |
3 | tubulin, beta, 2 | 5174735 | 50 | 4.7 | 24 (68%) | 6702 |
4 | tubulin, beta 4 | 21361322 | 50 | 4.8 | 19 (57%) | 5071 |
5 | glyceraldehyde-3-phosphate dehydrogenase, spermatogenic | 7657116 | 44 | 8.4 | 12 (48%) | 5711 |
6 | tubulin, alpha 3c | 17921993 | 50 | 4.9 | 15 (51%) | 2942 |
7 | ropporin, rhophilin associated protein 1B | 59891409 | 24 | 5.1 | 7 (56%) | 2587 |
8 | semenogelin II precursor | 4506885 | 65 | 9.1 | 13 (22%) | 2277 |
9 | outer dense fiber of sperm tails 2 isoform 2 | 24430183 | 73 | 7.2 | 16 (31%) | 2051 |
10 | semenogelin I isoform a preproprotein | 4506883 | 52 | 9.3 | 12 (31%) | 1983 |
11 | sperm protein associated with the nucleus, X chromosome, family member C | 13435137 | 11 | 5.0 | 3 (45%) | 1892 |
12 | tubulin, alpha, ubiquitous | 57013276 | 50 | 4.9 | 13 (46%) | 1616 |
13 | glutathione S-transferase mu 3 | 23065552 | 26 | 5.3 | 7 (37%) | 1595 |
14 | prolactin-induced protein | 4505821 | 16 | 8.2 | 8 (62%) | 1559 |
15 | A Kinase anchor protein 3 | 21493041 | 95 | 5.8 | 20 (35%) | 1416 |
16 | histone cluster 1, H2ba | 24586679 | 14 | 10.3 | 1 (11%) | 1371 |
17 | mitochondrial malate dehydrogenase precursor | 21735621 | 35 | 8.9 | 8 (33%) | 1342 |
18 | histone cluster 1, H2aa | 25092737 | 14 | 10.8 | 6 (58%) | 1334 |
19 | clusterin isoform 1 | 42716297 | 58 | 6.2 | 6 (16%) | 1204 |
20 | ropporin | 21359920 | 24 | 5.5 | 6 (43%) | 1184 |
21 | sorbitol dehydrogenase | 156627571 | 38 | 8.2 | 7 (35%) | 1095 |
22 | mitochondrial ATP synthase beta subunit precursor | 32189394 | 56 | 5.2 | 16 (53%) | 976 |
23 | beta actin | 4501885 | 42 | 5.2 | 9 (54%) | 909 |
24 | triosephosphate isomerase 1 isoform 1 | 4507645 | 26 | 6.4 | 9 (55%) | 761 |
25 | glyceraldehyde-3-phosphate dehydrogenase | 7669492 | 36 | 8.5 | 6 (32%) | 706 |
26 | L-lactate dehydrogenase C– 1 peptide | 4504973 | 36 | 7.0 | 1 (5%) | 674 |
27 | fatty acid synthase | 41872631 | 275 | 6.0 | 3 (8%) | 669 |
28 | sperm autoantigenic protein 17 | 8394343 | 17 | 4.7 | 2 (22%) | 592 |
29 | pyruvate kinase, muscle isoform M2 | 33286418 | 58 | 7.9 | 11 (38%) | 560 |
30 | ATP synthase, H + transporting, mitochondrial F1 complex, alpha subunit precursor | 4757810 | 59 | 9.1 | 8 (23%) | 542 |
31 | brain creatine kinase | 21536286 | 42 | 5.3 | 7 (32%) | 511 |
32 | heat shock protein 90Â kDa beta, member 1 | 4507677 | 92 | 4.7 | 6 (10%) | 502 |
33 | heat shock 70Â kDa protein 2 | 13676857 | 70 | 5.5 | 10 (21%) | 448 |
34 | heat shock 90Â kDa protein 1, alpha isoform 1 | 153792590 | 98 | 5.0 | 8 (13%) | 447 |
35 | saccharopine dehydrogenase (putative) | 55770836 | 47 | 9.2 | 5 (29%) | 395 |
36 | actin, alpha 1, skeletal muscle | 4501881 | 42 | 5.2 | 6 (36%) | 374 |
37 | glutamine synthetase | 19923206 | 42 | 6.4 | 3 (9%) | 374 |
38 | fructose-bisphosphate aldolase A | 4557305 | 39 | 8.3 | 5 (29%) | 366 |
39 | eukaryotic translation elongation factor 1 alpha 1 | 4503471 | 50 | 9.1 | 8 (36%) | 347 |
40 | fibronectin 1 isoform 3 preproprotein | 16933542 | 262 | 5.4 | 6 (4%) | 321 |
41 | prostate, ovary, testis expressed protein on chromosome 2 | 153791352 | 123 | 5.8 | 4 (8%) | 317 |
42 | acetyl-Coenzyme A acetyltransferase 1 precursor | 4557237 | 45 | 8.9 | 9 (33%) | 316 |
43 | voltage-dependent anion channel 2 | 42476281 | 32 | 7.4 | 4 (22%) | 293 |
44 | outer dense fiber of sperm tails 1 | 194248060 | 30 | 8.4 | 4 (26%) | 283 |
45 | phosphoglycerate kinase 2 | 31543397 | 45 | 8.7 | 5 (30%) | 253 |
46 | histone cluster 1, H2ae | 10645195 | 14 | 11.0 | 4 (49%) | 251 |
47 | protein disulfide – isomerase A3 precursor | 21361657 | 57 | 5.9 | 4 (13%) | 243 |
48 | acid phosphatase, prostate short isoform precursor | 6382064 | 44 | 5.8 | 5 (16%) | 240 |
49 | voltage-dependent anion channel 3 isoform b | 25188179 | 30 | 8.8 | 5 (27%) | 226 |
50 | phospholipase A2, group IIA precursor | 4505849 | 16 | 9.4 | 2 (23%) | 202 |
51 | transglutaminase 4 (prostate) | 156627577 | 77 | 6.3 | 8 (17%) | 187 |
52 | glutathione peroxidase 4 isoform A precursor | 75709200 | 22 | 8.6 | 4 (32%) | 181 |
53 | heat shock 70Â kDa protein 5 | 16507237 | 72 | 5.0 | 4 (8%) | 161 |
54 | heat shock protein beta-1 | 4504517 | 22 | 5.9 | 3 (31%) | 160 |
55 | angiotensin I converting enzyme 1 isoform 1 precursor | 4503273 | 150 | 5.9 | 3 (4%) | 160 |
56 | peroxiredoxin 6 | 4758638 | 25 | 6.0 | 2 (16%) | 145 |
57 | chaperonin containing TCP1, subunit 5 (epsilon) | 24307939 | 60 | 5.4 | 4 (12%) | 142 |
58 | valosin-containing protein | 6005942 | 89 | 5.1 | 3 (7%) | 142 |
59 | sperm acrosomal membrane protein 14 – 1 peptide | 19424138 | 13 | 5.4 | 1 (11%) | 132 |
60 | chaperonin containing TCP1, subunit 8 (theta) | 48762932 | 60 | 5.4 | 4 (14%) | 131 |
61 | L-lactate dehydrogenase A isoform 1 | 5031857 | 36 | 8.4 | 2 (13%) | 124 |
62 | phosphoglycerate dehydrogenase | 23308577 | 57 | 6.2 | 3 (13%) | 112 |
63 | clathrin heavy chain 1 | 4758012 | 193 | 5.4 | 4 (5%) | 108 |
64 | chaperonin containing TCP1, subunit 4 (delta) | 38455427 | 58 | 7.9 | 5 (21%) | 99 |
65 | peptidylprolyl isomerase A | 10863927 | 18 | 7.6 | 2 (23%) | 96 |
66 | fumarate hydratase precursor | 19743875 | 54 | 8.8 | 8 (31%) | 92 |
67 | RAB2A, member RAS oncogene family | 4506365 | 23 | 6.0 | 3 (18%) | 92 |
68 | olfactomedin 4 precursor | 32313593 | 57 | 5.5 | 2 (5%) | 90 |