Densitometric analysis of the protein levels of steroidogenic enzymes CYP11A1, 3βHSD, CYP17A1, 17βHSD, and CYP19 in A) small, B) medium and C) large ovarian follicles. Proteins were densitometrically scanned and normalized against β-actin. Western blot experiments were repeated three independent times using samples from three different animals. Data were plotted as mean±S.E.M. Statistical significance is indicated by * P < 0.05.