Effects of recombinant human resistin (0.1, 1, 10, and 100 ng/ml) on the mRNA expression of steroidogenic enzymes (i.e., CYP11A1, 3βHSD, CYP17A1, 17βHSD, and CYP19A1 ) in A) small, B) medium and C) large ovarian follicles. The expression of mRNA has been determined by real time-PCR. The expression of each gene was normalized to the expression of GAPDH. Real time PCR experiments were performed three independent times. In each experiment six ovaries from three different animals were selected. Data were plotted as mean±S.E.M. Statistical significance is indicated by * P < 0.05.