Figure 1

Resistin expression and concentration in ovarian follicles. Resistin expression and concentration in ovarian follicles collected from prepubertal animals; A) The ratio of resistin gene intensity to GAPDH intensity was used to represent the abundance of resistin mRNA in the ovarian follicles. B) The amount of protein (50 μg) in every sample was checked by immunoblot using anti-β-actin antibody. Upper panels: representative blots of tissue homogenates resolved by 15% SDS–PAGE, immunoblotted with resistin antibodies and reprobed with β-actin antiserum. Lower panels: results of densitometric quantification of bands. The data are expressed as the mean±SEM of rations relative to GAPDH or to β-actin. C) For positive control we used porcine white adipose tissue. D) Concentrations of resistin in follicular fluid and ovarian tissue. Different letters indicate statistically significant differences among groups (P < 0.05). E) Estradiol concentrations in follicular fluid.