Compound E inhibition of Notch signaling increased vascular smooth muscle cells (VSMC) density and altered VSMC organization in the ovarian stroma. A-D) Immunofluorescence staining (10× magnified) for Notch3 (green) and α-SMA (red). C, D) enlargement of boxes areas in upper panels (20× magnified). Please note increased thickness and disorganized appearance of VSMCs in B and D. E, F) PECAM (green) and α-SMA (red). Please note disorganisation and failure to overlap in F (20× magnified).