Placental TfR, DMT1+IRE and ferroportin expression. Placental expression of TfR protein (A), DMT1+IRE mRNA (B) and ferroportin protein (C) in Rowett Hooded Lister (RHL) and Wistar rats exposed to a control or low iron diet prior to and during pregnancy. Data are presented as means ± s.e.m. for n = 15–21 per group. Protein expression is normalised to tubulin expression and mRNA expression is normalised to β-actin expression. Representative sample blots are shown.