Figure 1From: Maternal diabetes causes abnormal dynamic changes of endoplasmic reticulum during mouse oocyte maturation and early embryo developmentSchematic illustration of diabetes induction, insulin treatment, and oocyte collection. Female mice received an injection of 190 mg/kg STZ on d 0. Four days later, blood glucose levels were determined via a commercial glucometer. Mice exhibiting blood glucose levels of at least 300 mg/dl were considered to be diabetic and then were administrated with 10 IU PMSG (d 4). Two days after PMSG, the ovaries were isolated, and cumulus-enclosed GV oocytes were collected (d 6). To retrieve Pro-MII and ovulated oocytes, mice were injected with 10 IU hCG 2 d after PMSG. At 8 h and 13.5 h of hCG administration, oocytes were collected from ovaries and oviductal ampullae (d 7), respectively.Back to article page