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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Maternal diabetes causes abnormal dynamic changes of endoplasmic reticulum during mouse oocyte maturation and early embryo development

Figure 1

Schematic illustration of diabetes induction, insulin treatment, and oocyte collection. Female mice received an injection of 190 mg/kg STZ on d 0. Four days later, blood glucose levels were determined via a commercial glucometer. Mice exhibiting blood glucose levels of at least 300 mg/dl were considered to be diabetic and then were administrated with 10 IU PMSG (d 4). Two days after PMSG, the ovaries were isolated, and cumulus-enclosed GV oocytes were collected (d 6). To retrieve Pro-MII and ovulated oocytes, mice were injected with 10 IU hCG 2 d after PMSG. At 8 h and 13.5 h of hCG administration, oocytes were collected from ovaries and oviductal ampullae (d 7), respectively.

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