Effect of 8-Br-cAMP and CRH on ERVW - 1 mRNA expression. BeWo cells were cultured in 10% CCS media and treated with or without 50~100 μM 8-Br-cAMP (A) or 10~100 nM CRH (B) for 48 h, the expression of ERVW-1 mRNA was determined by RT-quantitative PCR. Results shown are the induction fold relative to the control which was set at 1.0. Values are mean ± S.D. from 3 independent experiments. * p<0.05.