Effect of 8-Br-cAMP and CRH on cell apoptosis. BeWo cells were cultured in 10% CCS media and treated without or with 100 μM 8-Br-cAMP or 100 nM CRH for 72 h. (A) DAPI stain of apoptotic nuclei. Cells were fixed and nuclear morphology were observed by staining with DAPI and visualized by fluorescence microscopy (20×). Data shown are representative results from 3 independent experiments. Arrows shown are the condensed nuclei or nuclear fragments described in the text. (B) FACS analysis of apoptotic portion. Cells were collected, stained by Annexin V/7-ADD and measured by flow cytometry. Data shown is representative of FACS analysis. (C) Statistic analysis of apoptotic cells based on Figure 3B. Data were obtained from three independent experiments. *p<0.05.