8-Br-cAMP induced CRH and CRHR mRNA expression in BeWo cells. BeWo cells were cultured in 10% CCS media and treated without or with 100~1500 μM 8-Br-cAMP for 8~48 h, the expressions of CRH (A, B) CRHR1 and CRHR2 (C, D) mRNA were determined by RT-quantitative PCR. Results shown are the induction fold relative to the control which was set at 1.0. Values are mean ± S.D. from 3 independent experiments. * p<0.05, ** p<0.01.