Figure 3

Electron micrographs of muscular layer of vas deferens of animals from CG (A and B) and CMTG (C and D). In A and B, the smooth muscle cells (M) show nucleus (Nu) with regularly distributed chromatin and cytoplasm with numerous mitochondria (mi), miofilaments (Mf) and dense bodies (arrows). In high magnification, miofilaments (Mf) associated to dense bodies (arrows) are observed. In C, a cross-sectioned smooth muscle cell with numerous miofilaments (Mf) and dense bodies (arrows) is observed. Note that the cytoplasm is apparently more electron dense than in CG; the nucleus shows portions of strongly condensed chromatin (asterisks). In D, the shrunken cytoplasm of a smooth muscle cell (M1) containing dense bodies (white arrows) is more electron dense than CG. The nucleus is irregularly outlined and shows strongly electron dense chromatin in the nuclear periphery (asterisks). Next to this cell, two smooth muscle cells (M2 and M3) with irregular nuclei and portions of chromatin irregularly distributed in the nuclear periphery (arrowheads) are observed. A portion of strongly condensed chromatin is observed close to these nuclei (thick arrow). Bars: 4 μm (A); 1 μm (A, inset); 2.5 μm (B); 2 μm (C and D).