Figure 2

Photomicrographs of vas deferens of animals from CG (A, E, F, I) and CMTG (B-D, G-H, J) submitted to TUNEL method (A-D), NF-kB (E-H) and AR (I, J) immunohistochemistry and counterstained by hematoxylin. In A, no TUNEL-labeled cells are observed in the vas deferens. However, in C, TUNEL-positive nuclei are observed in the columnar (thick arrow) and mainly in the basal (thin arrows) cells of epithelium (EP). Lamina propria (LP). In B and D, numerous TUNEL-positive muscle cells are also observed in the muscular layer (arrows). In E and F (CG), portions of the muscular layer show weak (E, arrows) or no (F; asterisks) NF-kB immunolabeling while in G and H (CMTG), a strong immunostaining is noted in the cytoplasm of muscle cells (arrows). In I and J, note the strong (I) and weak (J) AR immunolabeling in the muscle cells of vas deferens of rats from CG and CMTG, respectively. Bars: 50 μm (A); 20 μm (B); 27 μm (C); 12 μm (D); 156 μm (E, G); 38 μm (F, H); 58 μm (I,J).