Table 1 Primers used in real-time qPCR analysis
Gene name | Gene symbol | Primer sequence (5’-3’) | Annealing temperature (°C) | Band (bp) | GenBank accession no. |
|---|---|---|---|---|---|
Beta actin | Actb | F: ctggcaccacaccttctac | 55.0 | 238 | NM_007393 |
R: gggcacagtgtgggtgac | |||||
Cytochrome P450, family 11, subfamily A, polypeptide 1 | Cyp11a1 | F: agatcccttcccctggcgacaatg | 60.0 | 192 | NM_019779 |
R: cgcatgagaagagtatcgacgcatc | |||||
Cytochrome P450, family 17, subfamily A, polypeptide 1 | Cyp17a1 | F: ccaggacccaagtgtgttct | 56.0 | 250 | NM_007809 |
R: cctgatacgaagcacttctcg | |||||
Cytochrome P450, family 19, subfamily A, polypeptide 1 | Cyp19a1 | F: catggtcccggaaactgtga | 56.0 | 187 | NM_007810 |
R: gtagtagttgcaggcacttc | |||||
Follicle stimulating hormone receptor | Fshr | F: gcagatgtgttctccaacctacc | 61.0 | 172 | NM_013523 |
R: ggagagactggatcttgtgaaagg | |||||
Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 | Hsd3b1 | F: caggagaaagaactgcaggaggtc | 59.5 | 280 | NM_008293 |
R: gcacacttgcttgaacacaggc | |||||
Steroidogenic acute regulatory protein | Star | F: cagggagaggtggctatgca | 57.0 | 262 | NM_011485 |
R: ccgtgtcttttccaatcctctg | |||||
Inhibin, beta-a | Inhba | F: tcacctttgccgagtcaggc | 59.0 | 97 | NM_008380 |
R: ccacacttctgcacgctcca |