Figure 4

Comparison of Inhba mRNA levels in response to FSH treatment in WT and AHRKO follicles. Early antral follicles from pre-pubertal WT and AHRKO ovaries were isolated and cultured in supplemented media in the presence of 0–15 IU/mL FSH for 7 days. At the end of culture, follicles were pooled per treatment group and genotype, subjected to extraction of RNA and then processed for qPCR analysis of Inhba, which is expressed as a mean relative expression ratio normalized to Actb as a loading control. Each bar represents the mean ± SEM (n=3-6 mice per genotype and 10–16 follicles at each selected treatment). The letter “a” above the bars indicates significant difference (p ≤ 0.05) from WT follicles within the same FSH treatment, and the letter “b” above the bar indicates significant difference (p ≤ 0.05) from 0 IU/mL FSH in the same genotype, using one-way ANOVA followed by Tukey’s test as a post hoc test.