Experimental day | Experimental procedure |
---|---|
0 | Transfer of low-altitude native sheep to a high altitude experimental station and formation of the experimental groups (HH: n=6; HHV: n=6; LH: n=6; LHV: n=6; LL: n=6; LLV: n=6) |
5 | Start treatment with vitamins C and E in groups HHV, LHV and LL |
5 | Surgery for installation of the arterial and venous catheters |
6 | Administration of the first dose of cloprostenol for estrus synchronization |
15 | Administration of the second dose of cloprostenol for estrus synchronization |
15 | Introduction of vasectomized males for detection of females in estrus |
17-18 | First estrus after synchronization treatment |
34-36 | Second estrus after synchronization treatment |
34-54 | Daily sampling of arterial blood for evaluation of oxygenation status and venous blood for the measurement of progesterone and biomarkers of oxidative stress (n=6 each group) |
34-54 | Daily ultrasound examinations for evaluation of preovulatory follicle size and corpus luteum growth (n=6 each group) |
51-54 | Third estrus after synchronization treatment |
56-59 | Laparoscopic removal of corpora lutea for assessment of growth factors and vascularization (n=6 each group) |