Figure 4

Luteal expression of insulin-like growth factors in cycling sheep at a high altitude. IGF-I (panel A) and IGF-II (panel B) mRNA expression (2^-ΔΔCt) in luteal tissue obtained at day 5 after ovulation in non-conceptional reproductive cycles in sheep at a high altitude that were either unsupplemented (HH: high-altitude natives; LH: low-altitude natives) or supplemented daily (HHV: high-altitude natives; LHV: low-altitude natives) with vitamins C and E. The LLV group corresponds to control cycling sheep at a low altitude with vitamin supplementation. The values of mRNA expression in each group are expressed relative to the control group LL (low-altitude native ewes maintained at a low altitude). Asterisks indicate significant differences (*= P<0.01 and ** = P<0.001) when compared to the control LL group (Dunn`s multiple comparison test).