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Table 5 Number of cells in the inner cell mass (ICM), trophectoderm (TE), ICM:TE ratio and total cell number in control, full-thickness and quarter zonal thinning groups in experiment 3

From: Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers

 

Control a

Full-thickness a

Thinning

P b

(n=62)

n=99)

(n=69)

ICM

23.7±3.4

20.85±3.08

21.63±3.06

0.000 c

TE

65.9±9.9

55.19±10.19

58.76±8.67

0.000 d

ICM:TE

0.36±0.04

0.38±0.05

0.36±0.03

0.02

Total

89.4±12.7

76.0±12.8

80.4±11.3

0.000 e

  1. Values are mean ± standard deviation (SD).
  2. a Some blastocysts were excluded due to accidental lost or poor staining.
  3. b As a safeguard against multiple tests of statistical significance on the same data, the Bonferroni correction was used to adjust the P-value. In this case, a P-value <0.05/4 or <0.0125 was considered to indicate a significant difference.
  4. One-way ANOVA with Scheffe post-hoc test when P<0.0125.
  5. c control vs. full-thickness group (P=0.000), and control vs. quarter-thinning group (P=0.001), and full-thickness vs. quarter thinning group (P=0.307).
  6. d control vs. full-thickness group (P=0.000), control vs. quarter thinning group (P=0.000), full-thickness vs. quarter-thinning group (P=0.076).
  7. e control vs. full-thickness group (P=0.000), control vs. quarter thinning group (P=0.000) full-thickness vs. quarter thinning group (P=0.09).